Table 2.
BACTH analysis of hetero-oligomerization of the Inc proteins.
| Bait protein T25 fusion | MWa (Da) | Developmental expressionb | Prey protein T18 fusion |
|---|---|---|---|
| Ct005 | 39537 | early | Ct005, IncA, IncF |
| Ct058 | 40072 | Mid | IncD |
| Ct101 | 17645 | Mid | NO |
| Ct115 incD | 14912 | early | IncD, Ct058, Ct222 |
| Ct116 incE | 13539 | early | NO |
| Ct117 incF | 10420 | early | IncF, IncA, IncC, IncD, IncG, Ct005, Ct058, Ct249, Ct850 |
| Ct118 incG | 17389 | early | IncD |
| Ct119 incA | 30313 | Mid | IncA, IncC, IncD, IncF, Ct005 |
| Ct134 | 15104 | NT | NO |
| Ct135 | 38477 | early | NO |
| Ct222 | 13915 | Mid | Ct222, IncD, Ct223, Ct224, Ct850 |
| Ct223 | 29475 | Mid | Ct223 |
| Ct225 | 13264 | Mid | Ct225 |
| Ct227 | 14155 | Mid | NO |
| Ct229 | 23422 | early | IncD, Ct222, Ct223 |
| Ct232 incB | 12237 | early | NO |
| Ct233 incC | 18418 | early | IncC, IncA |
| Ct249 | 12210 | early | Ct249, IncF |
| Ct813 | 29570 | Mid | Ct813 |
| Ct850 | 45822 | Mid | IncF, Ct222 |
The interactions identified with the BACTH system are listed in this table. All Inc proteins were fused to the C-terminus of T25 or T18. An interaction corresponds at a β-galactosidase activity of the DHT1 bacteria at least 5 times superior to the negative control (see Table S5). NO means that we did not find any blue clones (no interaction detected) on the plate. The homotypic interactions are in bold.
a
Deduced from protein sequence.
b
Developmental expression pattern as deduced from qPCR analysis.