FIGURE 3.

Relative fitness (selection rate, r) in three environments of mutants grouped by their suppression mutation class: wild-type (WT), two-component transcriptional regulator (1436), wrinkly-spreader operon (wsp), and putative polysaccharide production associated (PPA) genes. Note that mutation class PPA has been split for the ‘sup’ environment to distinguish between populations by duration of prior biofilm selection: ISN₃ (intermediate) compared to LSN₃ (late). Tukey post hoc tests were used for pairwise comparisons, with unique letters reflecting significant differences. Error bars are 95% CI of the mean.