FIG 8 .

YB produces abundant eDNA in vitro. Parental WHT (white circles), YA (yellow squares), and YB (orange triangles) bacteria were grown in an in vitro stomach model. (A and B) At 0, 4, 8, 12, 16, 20, 24, and 48 h, the model growth system was assayed for CFU (A), or supernatants were analyzed by agarose gel electrophoresis for extracellular DNA (B): lane 1, WHT at 12 h; lane 2, YA at 12 h; lane 3, YB at 12 h; lane 4, WHT at 24 h; lane 5, YA at 24 h; lane 6, YB at 24 h; lane 7, WHT at 48 h; lane 8, YA at 48 h; lane 9, YB at 48 h (B, lower panel). The presence of DNA in supernatant fractions was confirmed by addition of DNase I. (C) Quantification of eDNA produced by WHT, YA, and YB was performed in the gel. (D) eDNA levels were converted to theoretical genomes, and the increase in extracellular genomes per CFU per hour was calculated for each time period in the time course. *** denotes statistical significance of P < 0.001.