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. Author manuscript; available in PMC: 2016 Feb 27.
Published in final edited form as: J Mol Biol. 2014 Sep 16;427(4):824–839. doi: 10.1016/j.jmb.2014.09.002

Figure 2.

Figure 2

Domain order and key residues affect nucleation. (A) Representative traces of actin polymerization assays monitored by pyrene fluorescence. Minimal nucleation construct variants (0.25 µM) were added to 4 µM actin. (B) Gain of function point mutations convert NaS3d into a construct as potent as Sc3d (Na[fs]S3d). The converse mutations in Sc3d (Sc[qe]3d) result in loss of activity. (A,B) The time until half-maximum polymerization (t1/2) reported is the average of three independent trials +/− standard deviations.