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. Author manuscript; available in PMC: 2016 Feb 27.
Published in final edited form as: J Mol Biol. 2014 Sep 16;427(4):824–839. doi: 10.1016/j.jmb.2014.09.002

Figure 4.

Figure 4

Inhibition of actin polymerization by WH2 domains does not correlate with actin binding affinity. (A) Representative traces of actin polymerization in the presence of the four wild-type Spir-WH2 domains group together despite very different affinities for actin. (B) Adding Linker 3 has little effect on Sc but markedly decreases inhibition by Sd. (C) Mutations in Na decrease inhibition, making it behave more like Sc and mutations in Sc increase inhibition, making it behave more like Na. (A–C) 3 µM WH2 added to 4 µM actin is shown in all cases.