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Effects of individual WH2 domains on actin nucleation. (A) Representative pyreneactin polymerization assays of different WH2 domain constructs (1.5 µM) added to actin (2 µM) during nucleation. Each trace is colored according to the actin binding affinity of the added WH2 domain according to the inset heat map (red is high affinity). If the effect on nucleation correlated to affinity, the traces would have a rainbow-like trend, but it does not.
