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. Author manuscript; available in PMC: 2016 Feb 27.
Published in final edited form as: J Mol Biol. 2014 Sep 16;427(4):824–839. doi: 10.1016/j.jmb.2014.09.002

Figure 6.

Figure 6

Elongation rates of actin determined by TIRF microscopy. (A) Example of seeded actin (red) and elongation (green) in the presence of Sc observed by TIRF. (B) Actin elongation rates in the presence of 1 µM of the indicated WH2 domains. The values in parentheses are the predicted concentrations of free actin, given the Kds determined in this study. Asterisk indicates statistical significance (p < 0.01) when compared with actin alone (n is the number of filaments analyzed for each case; error bars are one standard deviation).