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. Author manuscript; available in PMC: 2016 Feb 15.
Published in final edited form as: J Neurosci Methods. 2014 Dec 30;241:132–136. doi: 10.1016/j.jneumeth.2014.12.017

Figure 1.

Figure 1

Figure A shows the basic experimental setup configuration. Stimulating electrode 1 (S1) is inserted in stratum radiatum and stimulating electrode 2 (S2) is inserted in stratum oriens. A recording pipette is placed in stratum radiatum and different concentrations of the AMPA receptor antagonist CNQX are applied to the tissue surface via the multifunctional pipette (MFP). The field potential (fEPSP) in stratum radiatum induced by S1 is directly recorded by the recording pipette and the field potential in stratum oriens induced by S2 is also recorded by the (same) recording pipette but in reversed form (reversed fEPSP). Figure B demonstrates the application of CNQX with MFP at different distances from the recording pipette.