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. Author manuscript; available in PMC: 2015 Nov 13.
Published in final edited form as: Arch Med Res. 2014 Nov 13;45(8):744–752. doi: 10.1016/j.arcmed.2014.11.003

Figure 4.

Figure 4

Validation of Smad target gene changes by qRT-PCR. HBMEC were exposed to HIV particles at p24 levels of 30 ng/mL for 24 h followed by co-treatment with 1 μM Aβ (1–40) for 10 min. Selected cultures were pretreated with dynamin inhibitor dynasore (Dy, 50 μM) for 30 min followed by co-treatment with 1 μM Aβ (1–40) for 10 min. Real-time RT-PCR was conducted on custom 96-well RT2 Profiler PCR array (Qiagen) using the SYBRgreen technique. Expression of mRNA was calculated and analyzed by the comparative CT method and normalized to human GAPDH. Values are mean ± SEM, n = 5. *Statistically significant at p <0.05, ***Statistically significant at p <0.001.