Abstract
The nucleoside analogue 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole, a selective inhibitor of heterogeneous chromosomal RNA synthesis in salivary gland cells of the midge Chironomus tentans, blocks the initiation of transcription in Balbiani rings 1 and 2. The analogue seems to be without appreciable effect on the elongation of growing RNA chains and allows finished molecules to be detached from the chromosomal sites. The RNA chain growth rate, based on measurements of the time required for completion of synthesis of Balbiani ring 1 and 2 RNA molecules after addition of the inhibitor, was estimated to be around 25 nucleotides per second at 18 degrees.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berendes H. D. Synthetic activity of polytene chromosomes. Int Rev Cytol. 1973;35:61–116. doi: 10.1016/s0074-7696(08)60352-6. [DOI] [PubMed] [Google Scholar]
- CANNON G. B. CULTURE OF INSECT SALIVARY GLANDS IN A CHEMICALLY DEFINED MEDIUM. Science. 1964 Nov 20;146(3647):1063–1063. doi: 10.1126/science.146.3647.1063. [DOI] [PubMed] [Google Scholar]
- Daneholt B., Edström J. E., Egyházi E., Lambert B., Ringborg U. RNA synthesis in a Balbiani ring in Chironomus tentans salivary gland cells. Chromosoma. 1969;28(4):418–429. doi: 10.1007/BF00284258. [DOI] [PubMed] [Google Scholar]
- Daneholt B. Giant RNA transcript in a Balbiani ring. Nat New Biol. 1972 Dec 20;240(103):229–232. doi: 10.1038/newbio240229a0. [DOI] [PubMed] [Google Scholar]
- Daneholt B. Transcription in polytene chromosomes. Cell. 1975 Jan;4(1):1–9. doi: 10.1016/0092-8674(75)90127-0. [DOI] [PubMed] [Google Scholar]
- Egyhazi E. Actinomycin D and RNA transport. Nature. 1974 Jul 19;250(463):221–223. doi: 10.1038/250221a0. [DOI] [PubMed] [Google Scholar]
- Egyházi E. A tentative initiation inhibitor of chromosomal heterogeneous RNA synthesis. J Mol Biol. 1974 Mar 25;84(1):173–183. doi: 10.1016/0022-2836(74)90220-4. [DOI] [PubMed] [Google Scholar]
- Geiduschek E. P., Haselkorn R. Messenger RNA. Annu Rev Biochem. 1969;38:647–676. doi: 10.1146/annurev.bi.38.070169.003243. [DOI] [PubMed] [Google Scholar]
- Manor H., Goodman D., Stent G. S. RNA chain growth rates in Escherichia coli. J Mol Biol. 1969 Jan 14;39(1):1–29. doi: 10.1016/0022-2836(69)90329-5. [DOI] [PubMed] [Google Scholar]
- Ringborg U., Rydlander L. Nucleolar-derived ribonucleic acid in chromosomes, nuclear sap, and cytoplasm of Chironomus tentans salivary gland cells. J Cell Biol. 1971 Nov;51(21):355–368. doi: 10.1083/jcb.51.2.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rose J. K., Mosteller R. D., Yanofsky C. Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions. J Mol Biol. 1970 Aug;51(3):541–550. doi: 10.1016/0022-2836(70)90007-0. [DOI] [PubMed] [Google Scholar]