Figure 3.
IL-27 signaling alters cellular inflammation but not clearance of bacteria during S. aureus pneumonia. C57BL/6, WT and IL-27Rα−/− mice, were infected with 1x108 of S. aureus (ATTC 49775) for 24 hours. Bacterial colony counts in the lung were measured, n = 7 (A). Cellular inflammation was examined by total cells per milliliter in the BAL (B), cell differentials (C) and hematoxylin and eosin-stained lung sections that were quantified for lung inflammation by a pathologist (KMR and JFA) blinded to the groups (D), n = 7. *p < 0.05 IL-27Ra−/− versus WT mice. All data are reported as means ± SEM for each group by unpaired t test (A-C) and two-way analysis of variance with the Bonferroni’s post-test (D). Data are the combined results of two replicate experiments.