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Titration of 2,2’-dipyridyl concentration. A. salmonicida strain LFI1238 was grown in LB containing 1% NaCl to an optical density at 0.4 (600 nm). The culture was split into six individual flasks and supplemented with different concentrations of 2,2'-dipyridyl before growth was monitored for 44 hours. Culture treated with 50 μM 2,2'–dipyridyl showed a slight reduction in growth and this concentration was therefore used in all subsequent experiments.
