Table 1. HERC2 Fragment MS Analysis and HCIP Identification^a.

F1 1–1000		F2 950–1750		F3 1700–2700		F4 2600–3600		F5 3550–4500		F6 4421–4834
Gene	NWD	Gene	NWD	Gene	NWD	Gene	NWD	Gene	NWD	Gene	NWD
MUS81	4.24	ASAH1	6	ITPR2	8.49	BICD1	14.7	PHKA2	7.75	LCP1	18
CCDC22	4.24	MAVS	3.5	PTPN1	8.49	BICD2	14	ENPP1	6.93	SELO	15
AGAP3	4.24	CST4	3	GNA13	8.49	ZFYVE9	12.37	EIF3H	6.12	SPECC1L	10.39
ARHGEF5	3.97	SPTLC2	2.42	FNDC3A	6	CEP170	11.64	MIOS	5.66	SNX6	10.39
EME1	3.35	ZG16B	2.27	EPCAM	5.2	SNAPIN	8.49	PHKB	5.41	ARAF	10.39
HERC1	3.35	NCCRP1	2	NCOA4	4.08	UBE3A	6.39	EIF3A	5.33	TUBB3	8.16
KIF20A	3.18	HLA-A	1.98	MAVS	3	SLC30A9	6	PHKG2	5.29	RAF1	6.89
HPS3	3	HM13	1.75	LACTB	3	PCM1	5.29	OCRL	5.2	ABR	6.09
HPS5	2.12	VDAC2	1.75	NEK11	2.83	FHOD3	5.2	MTMR3	5.2	RER1	6
TUBA1C	2.1	KRT80	1.6	HLA-E	2.83	AKAP9	4.9	EIF3E	4.78	TXLNG	6
MLL4	2	CSTA	1.5	LMNB2	2	NIN	4.41	PHKA1	4.74	CBX2	6
RAB34	2	BLMH	1.21	TUBA1C	1.77	NUDCD2	4.24	WDR59	4.58	PRR14L	3
NEURL4	1.98	PSMB4	1.03	FTH1	1.73	SLC27A4	3.67	EIF3B	4.38	SENP1	3
ZDHHC17	1.73	PSMD1	1.11	MLEC	1.7	ASPM	3.46	EIF3L	4.04	ATP4A	3
EPHA7	1.73	PSMD11	1.02	PSMD1	1.66	PEX1	3.46	EIF3M	3.96	EXD2	2.83
ZBTB5	1.7	PELP1	1	LMNA	1.56	USP20	3.32	EIF4G1	3.81	SSX2IP	2.83
NUP153	1.7	TEX10	1	HM13	1.5	ETAA1	3	EIF3G	3.75	TUBA1C	2.83
GTF3C2	1.7			BIRC6	1.5	PRR14L	3	EIF3C	3.74	RNGTT	2.64
MLF2	1.7			C19orf2	1.5	RIC8A	3	EIF3K	3.47	CDC25C	2.12
ARNT	1.67			PPM1B	1.5	PHKG2	2.83	EIF3D	3.19	MYO9B	2.12
RNGTT	1.5			COPB2	1.37	SCO2	2.83	CTNNA1	3	SLC27A4	2.12
C10orf118	1.5			OBSL1	1.33	WDR62	2.6	CTNNB1	2.83	CXorf57	2.12
TRMT2A	1.5			COPG2	1.31	GNAS	2.6	WDR24	2.3	KIAA0889	2.12
VSIG7	1.5			COPG	1.29	TUBA1C	2.19	EIF3I	2.26	FLII	2.08
FDFT1	1.34			COPA	1.25	PHKB	2.12	GCC2	2.22	FASTKD5	2

^aThe 25 highest scoring HERC2 interactors for each fragment following immunoprecipitation, mass spectrometry, and CompPASS. The scores for each HCIP were generated at a 0.95 confidence threshold. The genes in bold indicate HCIPs with NWD ≥ 1 when using a 0.98 threshold. Genes in italics were identified as an HCIP with more than one HERC2 fragment.