Table 2. Surface-Associated Proteins of F. tularensis LVS.
| protein name | locusa | signal peptideb | PSORTb localization (score)c | sera reactivityd | previously identified as membrane (M), secreted (T), or surface (S) protein and reference |
|---|---|---|---|---|---|
| Group 1 Surface Immunogense | |||||
| outer membrane associated protein FopA1f | FTL_1328 | SpI | OM (9.93) | X,Y | M,21,23,47,48 T,35 S25,28 |
| acetyl-CoA carboxylase, biotin carboxyl carrier protein subunit (AccB)f | FTL_1592 | unknown | X,Y | M21,47 | |
| 50S ribosomal protein L7/L12 (RplL)f | FTL_1745 | unknown | X,Y | M21 | |
| elongation factor Tu (TufA)f | FTL_1751 | Cyto (9.97) | X,Y | M21 | |
| Group 2 and 3 Surface Immuogense | |||||
| lipoprotein (LpnA)f | FTL_0421 | SpII | OM (10.00) | X | M,21,23,47,49 S26 |
| outer membrane protein OmpHf | FTL_0536 | SpI | unknown | X | M37,47 |
| hypothetical proteinf | FTL_0617 | Cyto (8.96) | X | M,21,37,49 T,35,41 S25 | |
| 50S ribosomal protein L9 (RplI)f | FTL_1026 | Cyto (9.97) | X | T35 | |
| glyceraldehyde-3-phosphate dehydrogenase (GapA)f | FTL_1146 | Cyto (9.97) | X | T35,41 | |
| chaperone protein DnaKf | FTL_1191 | NC (SP) | Cyto (9.97) | Y | M,21,23,50 T,35,41 S25 |
| peroxidase/catalase (KatG)f | FTL_1504 | NC (SP) | Cyto (9.26) | Y | M,21,23,47,50 T35,41 |
| chitinase family 18 protein (ChiA)f | FTL_1521 | SpI | unknown | Y | M23 |
| chaperonin GroELf | FTL_1714 | Cyto (9.97) | Y | M,21,23,43 T,35,41 S25 | |
| Nonimmunoreactive Surface Proteins | |||||
| outer membrane protein | FTL_0009 | SpI | Peri (9.84) | M,21,37,47,49 T35 | |
| intracellular growth locus, subunit B (IglB)f | FTL_0112 | Cyto (9.97) | M50 | ||
| intracellular growth locus, subunit C (IglC)f | FTL_0113 | unknown | M,21,37 T51 | ||
| elongation factor G (FusA)f | FTL_0234 | Cyto (10.00) | |||
| heat shock protein 90 (HtpG)f | FTL_0267 | Cyto (9.97) | |||
| glutamate dehydrogenase (Gdh)f | FTL_0269 | unknown | M,37 T35 | ||
| OmpA family proteinf | FTL_0325 | SpII | unknown | M21,23,47 | |
| peptidoglycan-associated lipoproteinf | FTL_0336 | SpII | OM (10.00) | M23,47−49 | |
| hypothetical proteinf | FTL_0569 | SpI | unknown | ||
| AhpC/TSA family proteinf | FTL_1015 | NC (SP) | unknown | M,21 T35,41 | |
| lipoprotein (DsbG, FipB)f | FTL_1096 | SpII | unknown | M23,37,47−49,52 | |
| hypothetical protein | FTL_1225 | SpII | unknown | ||
| hypothetical protein | FTL_1494 | SpII | unknown | ||
| succinyl-CoA synthetase subunit beta (SucC)f | FTL_1553 | Cyto (8.96) | M,50 T41 | ||
| aconitate hydratase (AcnA)f | FTL_1772 | Cyto (9.97) | M21,37,50 | ||
| citrate synthase (GltA) | FTL_1789 | Cyto (9.97) | |||
| cell division protein FtsZf | FTL_1907 | Cyto (9.12) | M21,49 | ||
| 30S ribosomal protein S1 (RpsA)f | FTL_1912 | Cyto (9.97) | |||
NCBI reference sequence identification codes matching each loci are listed in Table S3 in the Supporting Information.
Signal peptide prediction; SpI, signal peptide cleaved by signal peptidase I; SpII, signal peptide cleaved by signal peptidase II; NC (SP), nonclassical signal peptide.
Subcellular localization predicted with PSORTb; Peri, periplasmic; Cyto, cytosol; OM, outer membrane; CM, cytoplasmic membrane.
X and Y designate proteins were reactive to sera from mice immunized with MPF and F. tularensis LVS vaccination, respectively.
Group 1 immunogens are jointly recognized by F. tularensis LVS immune sera, group 2 immunogens by F. tularensis LVS immune sera, and group 3 immunogens by MPF immune sera.
Indicates the protein was identified in the MPF by multidimensional LC–MS/MS or from MPF 2D-PAGE protein spots.