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. Author manuscript; available in PMC: 2015 Feb 11.
Published in final edited form as: Dev Biol. 2006 Apr 4;295(2):604–614. doi: 10.1016/j.ydbio.2006.03.041

Figure 1.

Figure 1

Detection of Active SFKs in zebrafish eggs and zygotes.

Particulate fractions were prepared from samples of 20 unfertilized eggs (UF) or zygotes collected 2.5 minutes after fertilization (F). Aliquots were either used directly for western blot or used for immunoprecipitation of Fyn kinase with the anti-Fyn-3 antibody (Santa Cruz Biotechnology, Santa Cruz, CA) directed against the N-terminus of Fyn kinase (Fyn-ippt). The blots were incubated with the clone 28 antibody at 1μg/ml (clone 28) or with normal mouse IgG as a control (Ctrl). Aliquots of the same samples were analyzed separately and probed with an affinity purified rabbit antibody to the phosphorylated C-terminal tyrosine ([pY]530) obtained from Biosource International, Camerillo, CA (#44-912). Bound antibody was detected with goat anti-mouse IgG or goat anti-rabbit IgG coupled to horseradish peroxidase and visualized by chemiluminescence.