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. Author manuscript; available in PMC: 2015 Feb 11.
Published in final edited form as: Dev Biol. 2006 Apr 4;295(2):604–614. doi: 10.1016/j.ydbio.2006.03.041

Figure 2.

Figure 2

Detection of active SFKs in the unfertilized egg.

Unfertilized eggs were fixed as described in Materials and Methods, treated with pronase and the chorion removed, then cut in half with a scalpel. The samples were then blocked, permeabilized and incubated with the clone 28 antibody (A) or control mouse IgG (B) at 2.5μg/ml followed by Alexa Fluor 488-anti-mouse IgG. Samples were imaged by confocal fluorescence (above) or by transmitted light (below). The cut surfaces of the eggs are indicated by the small arrows. Magnification is indicated by the bar which represents 100μm.