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. Author manuscript; available in PMC: 2015 Feb 11.
Published in final edited form as: Dev Biol. 2006 Apr 4;295(2):604–614. doi: 10.1016/j.ydbio.2006.03.041

Figure 4.

Figure 4

Effect of fertilization on the distribution of active Src-family PTKs in the zebrafish egg. Eggs were fixed before (A), and at 30 seconds (B), 2.5minutes (C), and 8 minutes (D) after insemination and the chorion was removed by dissection after protease treatment to ensure complete access for immunostaining. The eggs were immunolabelled with the clone 28 antibody as described in Figure 2. Conditions of illumination and signal amplification in the fluorescence channel were maintained at a constant level to allow comparison of immunofluorescence intensity fro egg to egg. Eggs were oriented with the animal pole toward the top and the micropylar region is indicated by arrows. Magnification is indicated by the bar which represents 100 μm.