Fig. 2.

TbRab7 RNAi with BSF and PCF trypanosomes. BSF and PCF TbRab7 RNAi cell lines were cultured without or with tetracycline to induce specific knockdown.

A. Northern blot analysis of TbRab7 transcript levels. A representative blot (10 μg total RNA/lane) was probed for TbRab7 transcript (top), stripped, and reprobed for tubulin transcript as a loading control (bottom). Mobilities of size markers are indicated (kb). Analyses were performed after 28 h (BSF) and 72 h (PCF) of induction. Signal levels were quantified by phosphorimaging.

B. Cell density over time was determined by haemocytometer and cells were adjusted to starting density daily. Data are presented as means ± SEM (n = 3). All subsequent RNAi phenotypic analyses reported herein were performed at 28 h in BSF cells (arrowhead).