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. 2014 Dec 18;33(3):1057–1063. doi: 10.3892/or.2014.3680

Figure 3.

Figure 3

Salin inhibits MMP9 expression by decreasing JunD and AP-1 activation. (A) The regulation of Salin on the MMP9 promoter and enhancer. Relative luciferase activities of pGL3-MMP9-WT (containing the potential enhancer element) and several mutant derivatives of the MMP9 enhancer region for all motifs (pGL3-MMP9-mNF-κB, pGL3-MMP9-mSp1, pGL3-MMP9-mAP1-1, pGL3-MMP9-mAP1-2 and pGL3-MMP9-mAP1-1+2) were transfected in HCCLM3 cells treated without or with Salin (5-20 μmol/l). The results revealed that the AP-1 site is critical for Salin-regulated MMP9 enhancer activity. (B) Western blot analysis indicated that the expression of the components of AP-1 (JunB and JunD) was regulated by Salin (5–20 μmol/l). *p<0.05 vs. the control, **p<0.01 vs. the control. These results are representative of three independent experiments.