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. Author manuscript; available in PMC: 2015 Feb 11.
Published in final edited form as: Methods Mol Biol. 2015;1276:241–261. doi: 10.1007/978-1-4939-2392-2_14

Figure 1.

Figure 1

Data showing the determination of the urea dependences of K1 and k2. A. Rate constants of formation of filter-retainable open complexes (βCR) are determined at 25°C in BBassocurea at 0 m urea at 38 and 1.3 nM active RNAP using Equation 2. B. Data obtained for αCR are fit to Equation 5 as a function of active [RNAP] to obtain fits for K1 and k2 at varying concentrations of urea. C. Values of K1 and k2 are plotted as functions of urea and GB to determine solute concentration dependences, or “m-values”. Figure reproduced from (3) with permission (ACS Publications).