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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Methods Mol Biol. 2015;1276:13–29. doi: 10.1007/978-1-4939-2392-2_2

Fig. 3. Sample purification of the E. coli RNAP.

Fig. 3

A variant with a deletion in the β′ jaw (β′ residues 1149–1190) was used. Samples of the cell lysate (L), Ni-NTA eluate (N; see section 4.3), heparin peak fractions (H; see section 4.4), and MonoQ peak fractions (H; see section 4.5) were analyzed by SDS-PAGE using a 4–12% NuPAGE® Bis-Tris pre-cast polyacrylamide gel (Life Technologies). Chromatography through the MonoQ column separates the core enzyme (left) from the holoenzyme (right). The lane marked M was loaded with the molecular weight markers; the positions of the RNAP subunits are indicated on the right.