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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Methods Mol Biol. 2015;1276:13–29. doi: 10.1007/978-1-4939-2392-2_2

Table 2.

Vectors for site-directed mutagenesis or expression of single E. coli RNAP subunits

Plasmid Subunit & AbR Features Origin & Resistance
pIA281 αWT
— 		T7P—rpoA; α subunit under control of the bacteriophage T7 gene 10 promoter; HindIII site in rpoA is removed pMB1 Ampicillin
pIA545 βWT
— 		Ptrc—His6-rpoB WT; N-terminally tagged β under control of IPTG-inducible Ptrc promoter; a silent MfeI @ 555 pMB1 Ampicillin
pIA178 βS531F
RifR 		Ptrc—His6-rpoB S531F; a rifampicin-resistant (RifR) S531F allele in the Ptrc—rpoB construct pMB1 Ampicillin
pIA223 βD516V
RifR 		Ptrc—His6-rpoB D516V; a RifR D516V (marked with a silent BsgI site) allele in the Ptrc—rpoB construct pMB1 Ampicillin
pIA661 β′ WT
—		 Ptrc—rpoC WT-His6; C-terminally tagged β′ under control of an IPTG-inducible Ptrc promoter; a silent EagI @ 675 pMB1 Ampicillin
pIA635 β′ S793F
StlR 		Ptrc—rpoC S793F-His6; a streptolydigin-resistant S793F (marked with a silent EcoRV site) allele in the Ptrc—rpoC construct pMB1 Ampicillin
pIA388 β′I774S
CBRR 		Ptrc—rpoC I774S-His6; a CBR-resistant I774S (marked with a silent EarI site) allele in the Ptrc—rpoC construct pMB1 Ampicillin
pIA839 ωWT
—		 PBAD—rpoZ; ω subunit under control of arabinose-inducible PBAD promoter P15A Chloramphenicol
pIA586 σWT
—		 T7P—His6-rpoD; N-terminally tagged σ70 subunit under control of the T7 gene 10 promoter pMB1 Kanamycin
pIA1127 σWT
—		 T7P—His6-rpoD; N-terminally tagged σ70 under control of the T7 gene 10 promoter; removable His6 tag pMB1 Kanamycin
pIA458 βC & β′N
—		 A fragment encompassing the C-terminal part of rpoB, the linker with an SpeI site, and the N-terminal part of rpoC pUC Ampicillin