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. 2015 Jan 12;6:6047. doi: 10.1038/ncomms7047

Figure 2. Cellular specificity of amlexanox induced Il6 expression within fat.

Figure 2

(a) Il6 expression in mature adipocytes, SVC and whole inguinal adipose tissue (n=8 mice fractionated individually per treatment). (b) Relative Emr1 (F4/80, a macrophage marker) and (c) Adipoq (a mature adipocyte marker) expression in the different inguinal adipose tissue fractions (n=8 mice per treatment). (d,e) Gating strategy for fluorescence-activated cell sorting (FACS) of SVC. (f) Relative Emr1 and (g) Pdgfra (a preadipocyte marker) expression in different inguinal stromal cell fractions (n=4 groups of 2). (h) Il6 and (i) Tnfa expression in preadipocytes (CD45, CD31, Sca1+) and immune cells (CD45+ cells, both CD11c+ and CD11c populations) (n=4 separations per treatment each consisting of pooled inguinal fat from 2 mice). * indicates a P value<0.05 (heteroscedastic t-test) amlexanox versus vehicle. Error bars presented as s.e.m.