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. 2015 Jan 12;6:6047. doi: 10.1038/ncomms7047

Figure 5. Knockdown of p38 blocks amlexanox-stimulated Il6 expression in adipocytes.

Figure 5

Validated stealth RNAi to Mapk14 (1320001) and control (12935300) from Life Technologies was electroporated into 3T3-L1 adipocytes on day 1 of 10% FBS only media, and then 4 days after electroporation, vehicle and amlexanox treatments were administered after a 3-h serum starve. (a) Immunoblots for total and phosphorylated p38 protein. (b) quantification of immunoblots in a. (c) Further validation of p38 knockdown using Q-PCR to measure Mapk14 mRNA levels. (d) mRNA levels of Il6 after 4 h of amlexanox treatment in control and p38 knockdown cells (n=3 wells per condition). * indicates P value<0.05 p38 KD versus control, # indicates P value<0.05 amlexanox versus vehicle (two-way ANOVA plus Tukey–Krammer post-hoc analysis). Error bars represent s.e.m.