Table 2.
MS/MS peptide identification for tubulin spots. Data were processed using the MassLynx software package to generate peak list files (pkl) which were searched using Mascot. Mascot scores greater than 67 were considered significant (p<0.05).
| Sample | Protein ID | Species | Swiss Prot # | Mass | Mascot Score | # Peptides Identified |
|---|---|---|---|---|---|---|
| 5 mM glucose alpha tubulin spots | Tubulin alpha-2 chain | mouse | P05213 | 50152 | 208 | 8 (26%) |
| 30 mM glucose alpha tubulin spots | Tubulin alpha-2 chain | mouse | P05213 | 50152 | 250 | 7 (25%) |
| Tubulin alpha-1 chain | mouse | P68369 | 50136 | 110 | 7 (25%) | |
| 5 mM glucose beta tubulin spots | Tubulin beta-5 chain | mouse | P99024 | 49671 | 870 | 14 (37%) |
| Tubulin beta-2C chain | mouse | P68372 | 49831 | 753 | 14 (37%) | |
| Tubulin beta-2A chain | mouse | Q7TMM9 | 49907 | 632 | 12 (32%) | |
| Tubulin beta-3 chain | mouse | Q9ERD7 | 50419 | 512 | 10 (27%) | |
| Tubulin beta-6 chain | mouse | Q922F4 | 50090 | 345 | 8 (20%) | |
| 30 mM glucose beta tubulin spots | Tubulin beta-5 chain | mouse | P99024 | 49671 | 809 | 13 (33%) |
| Tubulin beta-2C chain | mouse | P68372 | 49831 | 739 | 13 (33%) | |
| Tubulin beta-3 chain | mouse | Q9ERD7 | 50419 | 575 | 9 (24%) | |
| Tubulin beta-2A chain | mouse | Q7TMM9 | 49907 | 573 | 10 (26%) | |
| Tubulin beta-6 chain | mouse | Q922F4 | 50090 | 416 | 7 (17%) |