Table III.
Summary of CHST11 gene expression and methylation status in human breast cancer cell lines tested.
| Cell line | ER status | Molecular subtype | CHST11 (qRT-PCR) | CS-A expression (2H6 mAb binding by flow cytometry) | DNA methylation status |
|---|---|---|---|---|---|
| MCF7 | Positive | Luminal | Low | Lowb | Hypermethylated |
| T47-D | Positive | Luminal | Low | Lowc | Hypermethylated |
| ZR-75-1 | Positive | Luminal | Low | Lowc | Hypermethylated |
| BT-474 | Positive | Her-2 amplified | Lowa | ND | Hypermethylated |
| MDA-MB-468 | Negative | Basal-like | Intermediate | Intermediate to highb | Hypomethylated |
| BT-20 | Negative | Basal-like | Intermediatea | ND | Hypomethylated |
| MDA-MB-231 | Negative | Basal-like | High | Highb | Hypomethylated |
| MDA-MET | Negative | Basal-like | High | Highb | Hypomethylated |
a
Digital gene expression with primers used to perform qRT-PCR was applied to estimate gene expression. ND, not determined.
b
Flow cytometry data were published before (14).
c
Flow cytometry was performed and no detectable binding of anti-CS-A mAb 2H6 was observed.