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. 2015 Feb 4;15(1):10. doi: 10.1186/s12862-015-0288-2

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© Rizzo et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Results of the short term experiments for the Pyrenean ( T. fonti ) and coastal ( T. ferreri ) species of Troglocharinus. A) Example graph of the lower thermal limit (LTL) experiment. The arrow marks the point at which the specimen froze (i.e. the supercooling point), with the sudden liberation of latent (or crystallization) heat, considered to be the LTL. B) Upper thermal limit (UTL). C) LTL. Columns, average temperature at which specimens died, with standard deviation.