Figure 6. AT2R-dependent cGMP production after photolysis of [Tyr(DMNB)⁴]Ang-II.

cGMP was measured in serum-starved HEK 293 cells transfected with AT2R after incubation with vehicle (control), Ang-II (10 nm) or [Tyr(DMNB)⁴]Ang-II, 10 nm) in the presence of PD123319 (PD, 1 μm), l-NAME (1 mm) and UV-irradiation, as indicated. Data are shown as the mean ± sem (n = 3 per condition). ***P < 0.001, ##P < 0.01 or ###P < 0.001 versus Ang-II, ^§§§P < 0.001 versus [Tyr(DMNB)⁴]Ang-II. ns, non-significant.