Figure 9. Photolysis of intracellular [Tyr(DMNB)4]Ang-II regulates transcription.
18S rRNA (A) and NF-κB mRNA (B) were quantified by quantitative PCR. A stimulation-control (scr) condition was performed where cardiomyocytes were loaded with a slowly-photolysing Ang-II analogue, Ang-II-ODMNB. Myocytes were incubated with [Tyr(DMNB)4]Ang-II or Ang-II-ODMNB (10 nm) for 30 min at room temperature. After incubation, cells were washed, placed on ice and exposed to a UV-lamp (30 W) for 1 min. In addition, cells were examined after treatment with buffer (Ctl) or extracellular Ang-II (Ang-II; 10 nm). Cells were then incubated at 37°C for 4 h prior to RNA extraction. Data are shown as the mean ± sem; *P < 0.05, **P < 0.01, ***P < 0.0001.