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. 2015 Jan 7;593(Pt 3):559–572. doi: 10.1113/jphysiol.2014.284182

Figure 1. Phosphorylation sites in the cytosolic domain of STIM1.

Figure 1

A, amino acid sequence of the cytosolic region of STIM1 indicating potential PKA-mediated serine and threonine phosphorylation sites, as determined by in silico prediction. The shaded area indicates those residues that would be absent in the STIM1-Δ448 construct that is terminated at residue His448. B, results of a representative in vitro PKA-mediated hyperphosphorylation assay. Shown are the effects of PKA activation on wild-type STIM1-Δ448 (WT versus WT + PKA), and the corresponding absence of any PKA effect in cells expressing STIM1-Δ448 bearing the T389A mutation (T389A + PKA).