Skip to main content
. 2015 Jan 7;593(Pt 3):559–572. doi: 10.1113/jphysiol.2014.284182

Figure 5. Effect of expression of an AKAP79 construct lacking the PKA-binding region on ARC channel currents.

Figure 5

A, mean (±SEM) inward ARC channel currents measured at −80 mV in cells expressing the Lck-STIM1-Δ448 construct with an AKAP79 construct lacking the PKA-binding domain (ΔPKA), or the same construct with a STIM1-Δ448 construct bearing the T389E mutation (ΔPKA/T389E). Values are mean ± SEM with values of n (number of cells) shown in parentheses. ***P < 0.0001 as indicated by Student's unpaired two-tailed t test. B, representative current–voltage relationships for the corresponding ARC channel currents in cells expressing the AKAP79-ΔPKA construct along with Lck-STIM1-Δ448 (black symbols), or the same construct along with a Lck-STIM1-Δ448 construct bearing the T389E mutation (grey symbols). C, representative Western blot demonstrating the co-immunoprecipitation of endogenous STIM1 with AKAP79.