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. 2014 Dec 18;593(Pt 3):601–618. doi: 10.1113/jphysiol.2014.283887

Figure 1. Uraemic serum up-regulates ILK activity in EA.hy926 cells and HUVECs.

Figure 1

A and B, EA.hy926 cells were incubated in medium supplemented with different normal serum (NS) or uraemic serum (US) concentrations for 24 h (A) or with 2.5% NS or US during different times (B). C, HUVECs were incubated in medium supplemented with 2.5% NS or 1–10% US for 24 h. Representative Western blots of phosphorylated GSK-3β in the serine-9 residue (P-GSK-3β) or ILK are shown. Total GSK-3β or GAPDH levels were determined as their respective endogenous controls. Bars represent the normalized densitometric analysis of the blots against the endogenous control values. All the values are represented as mean ± SEM of five independent experiments. *P < 0.05 vs. NS (CT serum-depleted cells, 24 h).