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. Author manuscript; available in PMC: 2015 Feb 11.
Published in final edited form as: J Hepatol. 2013 Jul 16;59(5):999–1006. doi: 10.1016/j.jhep.2013.07.010

Fig. 1. MV can infect BM-hMSCs and transfer the infection to HCC cells in vitro.

Fig. 1

(A) BM-hMSCs were infected with MV-GFP, (B) quantitated by flow cytometry for GFP-expressing cells and (C) evaluated for cell viability with MTS assay at the indicated time-points. CFSE-stained MV-infected BM-hMSCs were overlaid on DiI-labeled HuH7 cells at a 1:20 ratio. (D) At 48 and 72 hours post-overlaid, cells were photographed with a confocal microscope. (E) High-resolution analysis of heterofusion between green BM-hMSCs and red HuH7 cells resulted in observation of multinucleated yellow-colored synctia (Eii). For comparison, co-culture of uninfected BM-hMSCs (green) with HuH7 (red) did not result in heterofusion (Ei). Scale bar = 50 μm. (F) MV-induced syncytia formation increases with the number of infected BM-hMSCs. (This figure appears in colour on the web.)