Skip to main content
. Author manuscript; available in PMC: 2015 Feb 11.
Published in final edited form as: Acta Neuropathol. 2014 Feb 8;127(4):477–494. doi: 10.1007/s00401-014-1255-5

Fig. 5.

Fig. 5

A30P+P mice show elevated levels of calpain-resistant α-syn in the GL. a, c Representative sections of GL from A30P and A30P+P mice with and without treatment of calpain-1 and, b, d quantitative results. a After calpain1 digestion, brain sections were immunostained with either 15G7 antibody, displaying a calpain-cleavage site within its main epitope or c 105 antibody, with high preference to calpain-cleaved CT α-syn and immunoreactivity quantified by densitometry. Schematic diagrams (b, d) indicate antibody epitopes and location of a major calpain 1 cleavage site at aa 122. ANOVA revealed a 105 antibody-specific and significant accumulation of calpain-resistant α-syn in A30P+P mice, indicating accumulation of CT α-syn. The data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Ntg; §p < 0.05, §§p < 0.01 vs. A30P; +p < 0.05 A30P+calp vs. A30P+P+calp. Scale bar 25 μm