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. 2015 Feb 11;10(2):e0115644. doi: 10.1371/journal.pone.0115644

Fig 3. Protocol for Isolation of Labeled Proteome from Neuroblastoma Cell Lysate using Probe 1.

Fig 3

Cell lysate sample treated with probe 1 under UV was loaded on monomeric avidin column and washed with PBS to elute non-bound proteome, 2mM biotin to elute weakly bound proteome and 2.5M glycine (pH = 2.8) to elute strongly bound proteome.