Fig 5. Western Analysis representing reproducible enrichment of probe 1-labeled proteome by avidin chromatography.

Duplicate SDS-gels containing different washes from the avidin column loaded with N₁₈TG₂ were run where Lane 1 contains MW standards (M1), lanes 2–4 are the PBS Wash, lanes 5–7 are the Biotin Wash, lane 8–10 are the Regeneration Wash, and lane 11 contains biotinylated MW standards (M2). a) gel stained using Coomassie total protein stain and b) gel visualized via Western analysis through an anti-biotin antibody HRP conjugate.