Figure 2.

Metacyclogenesis as evaluated by flow cytometry. Parasites in growth logarithmic phase were distributed into 24-well plates (5 × 10⁵ parasites/mL) in Grace's medium, at 26°C, in triplicates. After 2, 6, or 10 day of culture, parasites were analyzed by flow cytometry. Figures show size (FSC) and granulocity (SSC) of parasites from 2nd ((a) IMG3 L. braziliensis; (b) MAB6 L. amazonensis), 6th ((c) IMG3; (d) MAB6), and 10th ((e) IMG3; (f) MAB6) days of culture. FSC^low are metacyclic forms (left gate) and FSC^high are procyclic forms (right gate). (g) and (h) data represent percentage of small forms (median with minimal and maximal values) of four independent experiments. ^* P < 0.05 (2nd versus 6th; 2nd versus 10th day).