Figure 5.
Metacyclogenesis as evaluated by complement resistance. Parasites in growth logarithmic phase were distributed into 24-well plates (5 × 105 parasites/mL) in Grace's medium, at 26°C, in triplicates. After 2, 6 or 10 days of culture parasites were incubated with 10% of rabbit serum and percentage of viable parasites (complement-resistant forms) was determined after 1 h of incubation, at 37°C. Data represent median and minimal and maximal values from 3 independent experiments. * P < 0.05 (2nd versus 6th; 2nd versus 10th day). # P < 0.05 (PPS6m versus M2903/IMG3, 2nd and 6th days).