Table 2. Tissue distribution of Wolbachia in Cubitermes castes and stages.

Castes	Tissues	12S rRNA gene	Wolbachia
Queen (n = 2)	Head	+	+
	Eggs	+	+
	Ovarian tissues	+	+
	Malpighian tubes	+	+
King (n = 2)	Head	+	+
	Fat tissues	+	+
Workers (n = 2)	Head	+	+
	Thorax + legs	+	+
White soldiers (n = 2)	Head	+	+
	Thorax + legs	+	+
Mature soldiers (n = 2)	Head	+	-
	Thorax + legs	+	+
Pool of 10 workers (n = 2)	Salivary glands	+	+

Queens were from T5 (C. spA) and T42 (C. spB), kings were from T38 (C. spA) and T42 (C. spB), workers, white soldiers and mature soldiers were from T42 (C. spB) and workers’ salivary glands were from T34 (C. sp A) and T42 (C. spB). The occurrence of Wolbachia was tested using wsp PCR and recorded as positive (+) or negative (-). The DNA quality was tested by using 12S ribosomal RNA gene.