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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Antiviral Res. 2014 Oct 31;113:49–61. doi: 10.1016/j.antiviral.2014.10.011

Figure 1. AD169IE2-YFP-infected cells express IE2-YFP as an immediate-early protein.

Figure 1

(A) Mock (lanes 1, 5, and 9) and AD169IE2-YFP-infected (lanes 2-4, 6-8, 10-12) (MOI:5) MRC5 cells were harvested up to 24 hpi and subjected to immunoblot analysis for CMV IE2 (lanes 1-4), CMV IE1 (lanes 5-8), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (lanes 9-12) polypeptides. The respective polypeptides and relative molecular mass markers are indicated. (B) Mock- and AD169IE2-YFP-infected MRC5 cells (MOI:3) were stained with Hoechst reagent (blue) and analyzed by fluorescent microscopy (×20) at 24hpi. Both the Hoechst reagent and IE2-YFP (green) are localized to the nucleus and the overlay highlights the virus-infected cells.