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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Antiviral Res. 2014 Oct 31;113:49–61. doi: 10.1016/j.antiviral.2014.10.011

Figure 5. Validation of selective hit compounds to inhibit a CMV infection.

Figure 5

MRC5 cells pre-treated with DMSO, podofilox (5-50nM), convallatoxin (5-50nM), or colchicine (10-500nM) were infected with AD169-WT (MOI:3) and total cell lysates were resolved on a SDS-polyacrylamide gel (12%). Mock (lanes 1 and 12) and infected (lanes 2-11, 13-22) cells were harvested at 20 hpi and subjected to immunoblot analysis for the early CMV gene product US11 (lanes 1-11) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (lanes 12-22). The respective polypeptides and relative molecular mass markers are indicated.