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. Author manuscript; available in PMC: 2016 Jan 6.
Published in final edited form as: Cell Rep. 2014 Dec 24;10(1):62–74. doi: 10.1016/j.celrep.2014.12.011

Figure 2. Responses to CNO are greater in Nkx2-1Cre mice than wild-type and Sf1Cre controls.

Figure 2

(A) cFOS immunoreactivity in the VMHVL of Nkx2-1Cre female, Nkx2-1Cre male, and wild-type female mice 70–75 min after CNO injection. cFOS+ cell numbers in one VMHVL from Nkx2-1Cre females (n=8) and wild-type females (n=5) analyzed 15–100 minutes after CNO injection, difference significant by two-tailed t-test and by Mann Whitney U test, scale bar = 100 μm.

(B) Average oxygen consumption normalized to lean mass and cumulative total movement, ambulatory movement, and food intake per animal (24–148 minutes, ~1.5 h) after saline (black) and CNO (grey) in wild-type females (n=5), Nkx2-1Cre females (n=8), wild-type males (n = 3), and Nkx2-1Cre males (n = 7). Fold-changes comparing the effects of CNO (Nkx2-1Cre(CNO/Saline) / WT (CNO/Saline)) are shown for significant pairwise comparisons. Pairwise comparisons of wild-type and Nkx2-1Cre mice treated with CNO were performed using Holm-Sidak multiple comparison tests following significant effects of genotype in a two-way ANOVA (p<0.05 for all comparisons shown except food intake), * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001. See also Figure S3.