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. Author manuscript; available in PMC: 2016 Jan 6.
Published in final edited form as: Cell Rep. 2014 Dec 24;10(1):62–74. doi: 10.1016/j.celrep.2014.12.011

Figure 4. Nkx2-1 ablation in the VMH results in female specific obesity but does not affect fertility.

Figure 4

(A) Photographs of adult Nkx2-1f/f and Nkx2-1f/f/Sf1Cre female mice.

(B) Body weight curves from Nkx2-1f/f/Sf1Cre (grey open circles, dashed lines) males and females compared to respective control littermates (black triangles, solid lines, n = 8–11/group).

(C, D) Mean body length, lean mass, and fat mass as determined by DEXA for Nkx2-1f/f/Sf1Cre (n = 7) or Nkx2-1f/f (n = 8) females at 22 weeks of age.

(E) Mean weights of dissected visceral, subcutaneous and BAT fat depots standardized to total body weight from Nkx2-1f/f/Sf1Cre (n = 5) or Nkx2-1f/f (n = 6) females at 22 weeks of age. Plasma leptin (ng/mL) from females analyzed during estrus (n = 2/group).

(F) Representative images of H&E staining of gonadal WAT (gWAT) and BAT in 30 week-old Nkx2-1f/f/Sf1Cre (n = 3) or Nkx2-1f/f (n = 2) females.

(G) Transcript expression in BAT from 10 week-old Nkx2-1f/f/Sf1Cre or Nkx2-1f/f females (n = 4/group).

(H) Pups per litter produced from mating male and female controls (Nkx2-1f/f), Nkx2-1f/f/Sf1Cre females with a control males or Nkx2-1f/f/Sf1Cre males with control females (n ≥ 14 litters/group). Plasma estradiol during estrus (pg/mL, n = 2/group), ovarian histology, and number of corpora lutea per section per ovary (n = 4 mice/group). See also Figure S5.