Fig. 2.

Inhibiting eCB signalling affects neuroblast directionality along the RMS.

Five to seven days after in vivo electroporation of pCX-EGFP in P2 mice, brain slices were cultured with vehicle or drugs for 2 h followed by time-lapse imaging for 3 h in the same medium. (A) A typical sagittal brain slice showing labelled neuroblasts. The two red rectangular boxes labelled 1 and 2 indicate the imaging sites in the beginning (caudal) and end (rostral) of RMS, respectively. Incubation with CB1 and CB2 antagonists (AM251 + JTE-907, 1 μM each), or with OMDM-188 (1 μM), significantly increased the percentage of exploratory neuroblasts at the expense of directed neuroblasts in both regions of the RMS (B, caudal RMS; C, rostral RMS). Graphs show mean ± s.e.m. (n = 6 brain slices for each condition, ~ 15–30 cells analysed per slice); *p < 0.05, **p < 0.01, ***p < 0.001. Bar, 400 μm.