Fig. 4.

Inhibiting BDNF signalling affects neuroblast directionality along the RMS.

Five days after in vivo electroporation of pCX-EGFP in P2 mice, brain slices were cultured with vehicle, TrkA-Fc or TrkB-Fc (1 μg/ml) for 2 h followed by time-lapse imaging for 3 h in the same medium. Neuroblast migration was filmed and analysed in the beginning and end of RMS (regions 1 and 2 in Fig. 2A). Incubation with TrkB-Fc significantly increased the percentage of exploratory neuroblasts at the expense of directed neuroblasts towards the beginning (A) and end of the RMS (B). Incubation with TrkA-Fc did not have any significant effect compared to control slices. Graphs show mean ± s.e.m. (n = 6–8 brain slices for each condition, ~ 15–30 cells analysed per slice); *p < 0.05, **p < 0.01, ***p < 0.001.