Fig. 5.

FGFR signalling regulates neuroblast migration in the RMS.

Time-lapse movies of GFP-labelled neuroblasts in the descending arm of the RMS from slices treated with different drugs targeting the FGFR signalling system (1 μM AZD4547 or 1 μg/ml FGFR1-Fc) were analysed using Volocity. Representative migratory tracks of 15 cells over a 3 hour filming period from a control (A), an AZD4547 (B) or a FGFR1-Fc (C) -treated brain slice. White stars mark the end point of each migration track, OB indicates the location of the olfactory bulb. Inhibiting FGF signalling increased the percentage of time spent immobile by neuroblasts (D), while significantly decreasing the percentage of cells migrating towards the OB (E) and the overall cell displacement (F). Graphs show mean ± s.e.m. (n = 6–7 brain slices for each condition, ~ 15–30 cells analysed per slice); *p < 0.05, **p < 0.01.