Fig. 6.

Targeting FGF signalling affects neuroblast migration in the beginning but not in the end of RMS.

Acute mouse brain slices containing GFP-labelled neuroblasts were prepared 5–7 days after in vivo electroporation of pCX-EGFP, cultured with vehicle or drugs for 2 h and imaged for 3 h in the same medium. Targeting the FGF signalling system using two distinct FGFR1 inhibitors (AZD4547 1 μM, or FGFR1-Fc 1 μg/ml), a FGF-2 neutralising antibody (bFM-1, 10 μg/ml), or FGF-2 (50 ng/ml) decreased the percentage of cells migrating in a directed fashion and increased the amount of exploratory neuroblasts in the beginning (A) but not in the end of RMS (B). Graphs show mean ± s.e.m. (n = 5–8 brain slices for each condition, ~ 15–30 cells analysed per slice); *p < 0.05, **p < 0.01, ***p < 0.001.