Table 2.

In vivo isomerase activity of EhPDI enzyme variants.

Enzyme variant	Acid phosphatase (AppA)∗ activity in Miller units (%)¶
EhPDISS/SS	732 ± 74 (−1)
EhPDISS/CC	1086 ± 136 (7)
EhPDICC/SS	1646 ± 230 (20)

^∗The data was expressed as ± standard error (n = 6).

^¶Under the background of ΔdsbC/pBAD-AppA, normalization was performed considering the mean activity of pBPelB-EhPDI as maximal (A _max⁡ = 5151) and pBluescript SK- as minimal (A _min⁡ = 780). The percentage (%) was calculated as [(A − A _min⁡)/(A _max⁡ − A _min⁡)] × 100, where A represents the activity of the enzyme variant.