Table 1.

Source, dilution, antigen retrieval method, and incubation time of different biomarkers.

Marker	Source	Host	Dilution	Antigen retrieval	Incubation time
Her-2	SP3 (Lab Vision, USA)	Rabbit	1 : 400	10 mM citrate buffer pH 6.0, pressure cooker	40 min, RT
Topo2α	Ki-SI (Lab Vision, USA)	Mouse	1 : 400	10 mM citrate buffer pH 6.0, pressure cooker	30 min, RT
P53	Y5 (Lab Vision, USA)	Rabbit	1 : 100	10 mM citrate buffer pH 6.0, pressure cooker	30 min, RT
Ki-67	SP6 (Lab Vision, USA)	Rabbit	1 : 200	10 mM citrate buffer pH 6.0, pressure cooker	30 min, RT