Figure 3.

The anti-EGFR Ab treatment and rapamycin reduce the expression of estrogen and progesterone receptors and Snail in pulmonary nodules. (a) Immunohistochemical analysis of lung nodules 30 weeks after cell administration shows a very high expression of estrogen and progesterone receptors (brown staining) which is decreased by anti-EGFR Ab and rapamycin. Scale bars: 10 μm. (b) Expression of Snail (brown staining) is inhibited by the two drugs in lung nodules. Scale bars: 50 μm. (c) Anti-EGFR Ab and rapamycin incubation for 24 hours reduce Snail level in LAM/TSC cells. β-Actin is evaluated as loading control. COS7 cells are used as control.